Multiplex Real-Time PCR Diagnostic of Relapsing Fevers in Africa
Identifieur interne : 004593 ( Main/Exploration ); précédent : 004592; suivant : 004594Multiplex Real-Time PCR Diagnostic of Relapsing Fevers in Africa
Auteurs : Haitham Elbir [France] ; Mireille Henry [France] ; Georges Diatta [Sénégal] ; Oleg Mediannikov [France, Sénégal] ; Cheikh Sokhna [Sénégal] ; Adama Tall [Sénégal] ; Cristina Socolovschi [France] ; Sally J. Cutler [Royaume-Uni] ; Kassahum D. Bilcha [Éthiopie] ; Jemal Ali [Éthiopie] ; Dayana Campelo [Australie] ; Steven C. Barker [Australie] ; Didier Raoult [France] ; Michel Drancourt [France]Source :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2013.
Abstract
In Africa, relapsing fever borreliae are neglected arthropod-borne pathogens causing mild to deadly septicemia and miscarriage. The closely related
We designed a multiplex real-time PCR protocol targeting the 16S rRNA gene detecting all four
The multiplex real-time PCR developed in this study is a rapid technique for both molecular detection and speciation of relapsing fever borreliae from blood in Africa. It could be incorporated in point-of-care laboratory to confirm diagnosis and provide evidence of the burden of infection attributed to different species of known or potentially novel relapsing fever borreliae.
Url:
DOI: 10.1371/journal.pntd.0002042
PubMed: 23390560
PubMed Central: 3561136
Affiliations:
- Australie, France, Royaume-Uni, Sénégal, Éthiopie
- Angleterre, Grand Londres, Provence-Alpes-Côte d'Azur
- Londres, Marseille
- Université d'Aix-Marseille
Links toward previous steps (curation, corpus...)
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- to stream Ncbi, to step Curation: 001130
- to stream Ncbi, to step Checkpoint: 001130
- to stream Main, to step Merge: 004693
- to stream Main, to step Curation: 004593
Le document en format XML
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</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix Marseille Université, URMITE, UMR63, CNRS 7278, IRD 198, Inserm 1095, Marseille</wicri:regionArea>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
<settlement type="city">Marseille</settlement>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS Neglected Tropical Diseases</title>
<idno type="ISSN">1935-2727</idno>
<idno type="eISSN">1935-2735</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>In Africa, relapsing fever borreliae are neglected arthropod-borne pathogens causing mild to deadly septicemia and miscarriage. The closely related <italic>Borrelia crocidurae, Borrelia duttonii, Borrelia recurrentis</italic>
and <italic>Borrelia hispanica</italic>
are rarely diagnosed at the species level, hampering refined epidemiological and clinical knowledge of the relapsing fevers. It would be hugely beneficial to have simultaneous detection and identification of <italic>Borrelia</italic>
to species level directly from clinical samples.</p>
</sec>
<sec><title>Methodology/Principal Findings</title>
<p>We designed a multiplex real-time PCR protocol targeting the 16S rRNA gene detecting all four <italic>Borrelia</italic>
, the <italic>glpQ</italic>
gene specifically detecting <italic>B. crocidurae</italic>
, the <italic>recN</italic>
gene specifically detecting <italic>B. duttonii/B. recurrentis</italic>
and the <italic>recC</italic>
gene specifically detecting <italic>B. hispanica</italic>
. Compared to combined 16S rRNA gene and <italic>flaB</italic>
gene sequencing as the gold standard, multiplex real-time PCR analyses of 171 <italic>Borrelia</italic>
-positive and 101 <italic>Borrelia</italic>
-negative control blood specimens yielded 100% sensitivity and specificity for <italic>B. duttonii/B. recurrentis</italic>
and <italic>B. hispanica</italic>
and 99% sensitivity and specificity for <italic>B. crocidurae</italic>
.</p>
</sec>
<sec><title>Conclusions/Significance</title>
<p>The multiplex real-time PCR developed in this study is a rapid technique for both molecular detection and speciation of relapsing fever borreliae from blood in Africa. It could be incorporated in point-of-care laboratory to confirm diagnosis and provide evidence of the burden of infection attributed to different species of known or potentially novel relapsing fever borreliae.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Vial, L" uniqKey="Vial L">L Vial</name>
</author>
<author><name sortKey="Diatta, G" uniqKey="Diatta G">G Diatta</name>
</author>
<author><name sortKey="Tall, A" uniqKey="Tall A">A Tall</name>
</author>
<author><name sortKey="Ba El, H" uniqKey="Ba El H">H Ba el</name>
</author>
<author><name sortKey="Bouganali, H" uniqKey="Bouganali H">H Bouganali</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fukunaga, M" uniqKey="Fukunaga M">M Fukunaga</name>
</author>
<author><name sortKey="Ushijima, Y" uniqKey="Ushijima Y">Y Ushijima</name>
</author>
<author><name sortKey="Aoki, Ly" uniqKey="Aoki L">LY Aoki</name>
</author>
<author><name sortKey="Talbert, A" uniqKey="Talbert A">A Talbert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kisinza, Wn" uniqKey="Kisinza W">WN Kisinza</name>
</author>
<author><name sortKey="Mccall, Pj" uniqKey="Mccall P">PJ McCall</name>
</author>
<author><name sortKey="Mitani, H" uniqKey="Mitani H">H Mitani</name>
</author>
<author><name sortKey="Talbert, A" uniqKey="Talbert A">A Talbert</name>
</author>
<author><name sortKey="Fukunaga, M" uniqKey="Fukunaga M">M Fukunaga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nordstrand, A" uniqKey="Nordstrand A">A Nordstrand</name>
</author>
<author><name sortKey="Bunikis, I" uniqKey="Bunikis I">I Bunikis</name>
</author>
<author><name sortKey="Larsson, C" uniqKey="Larsson C">C Larsson</name>
</author>
<author><name sortKey="Tsogbe, K" uniqKey="Tsogbe K">K Tsogbe</name>
</author>
<author><name sortKey="Schwan, Tg" uniqKey="Schwan T">TG Schwan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sarih, M" uniqKey="Sarih M">M Sarih</name>
</author>
<author><name sortKey="Garnier, M" uniqKey="Garnier M">M Garnier</name>
</author>
<author><name sortKey="Boudebouch, N" uniqKey="Boudebouch N">N Boudebouch</name>
</author>
<author><name sortKey="Bouattour, A" uniqKey="Bouattour A">A Bouattour</name>
</author>
<author><name sortKey="Rihani, A" uniqKey="Rihani A">A Rihani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouattour, A" uniqKey="Bouattour A">A Bouattour</name>
</author>
<author><name sortKey="Garnier, M" uniqKey="Garnier M">M Garnier</name>
</author>
<author><name sortKey="M Ghirbi, Y" uniqKey="M Ghirbi Y">Y M'Ghirbi</name>
</author>
<author><name sortKey="Sarih, M" uniqKey="Sarih M">M Sarih</name>
</author>
<author><name sortKey="Gern, L" uniqKey="Gern L">L Gern</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ramos, J" uniqKey="Ramos J">J Ramos</name>
</author>
<author><name sortKey="Malmierca, E" uniqKey="Malmierca E">E Malmierca</name>
</author>
<author><name sortKey="Reyes, F" uniqKey="Reyes F">F Reyes</name>
</author>
<author><name sortKey="Wolde, W" uniqKey="Wolde W">W Wolde</name>
</author>
<author><name sortKey="Galata, A" uniqKey="Galata A">A Galata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jongen, Vh" uniqKey="Jongen V">VH Jongen</name>
</author>
<author><name sortKey="Van Roosmalen, J" uniqKey="Van Roosmalen J">J van Roosmalen</name>
</author>
<author><name sortKey="Tiems, J" uniqKey="Tiems J">J Tiems</name>
</author>
<author><name sortKey="Van Holten, J" uniqKey="Van Holten J">J Van Holten</name>
</author>
<author><name sortKey="Wetsteyn, Jc" uniqKey="Wetsteyn J">JC Wetsteyn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tordini, G" uniqKey="Tordini G">G Tordini</name>
</author>
<author><name sortKey="Giaccherini, R" uniqKey="Giaccherini R">R Giaccherini</name>
</author>
<author><name sortKey="Corbisiero, R" uniqKey="Corbisiero R">R Corbisiero</name>
</author>
<author><name sortKey="Zanelli, G" uniqKey="Zanelli G">G Zanelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Million, M" uniqKey="Million M">M Million</name>
</author>
<author><name sortKey="Cazorla, C" uniqKey="Cazorla C">C Cazorla</name>
</author>
<author><name sortKey="Doudier, B" uniqKey="Doudier B">B Doudier</name>
</author>
<author><name sortKey="La Scola, B" uniqKey="La Scola B">B La Scola</name>
</author>
<author><name sortKey="Parola, P" uniqKey="Parola P">P Parola</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poirier, P" uniqKey="Poirier P">P Poirier</name>
</author>
<author><name sortKey="Lebuisson, A" uniqKey="Lebuisson A">A Lebuisson</name>
</author>
<author><name sortKey="Menager, C" uniqKey="Menager C">C Menager</name>
</author>
<author><name sortKey="Moulin, F" uniqKey="Moulin F">F Moulin</name>
</author>
<author><name sortKey="Dupouy Camet, J" uniqKey="Dupouy Camet J">J Dupouy-Camet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seboxa, T" uniqKey="Seboxa T">T Seboxa</name>
</author>
<author><name sortKey="Rahlenbeck, Si" uniqKey="Rahlenbeck S">SI Rahlenbeck</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dupont, Ht" uniqKey="Dupont H">HT Dupont</name>
</author>
<author><name sortKey="La Scola, B" uniqKey="La Scola B">B La Scola</name>
</author>
<author><name sortKey="Williams, R" uniqKey="Williams R">R Williams</name>
</author>
<author><name sortKey="Raoult, D" uniqKey="Raoult D">D Raoult</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parola, P" uniqKey="Parola P">P Parola</name>
</author>
<author><name sortKey="Diatta, G" uniqKey="Diatta G">G Diatta</name>
</author>
<author><name sortKey="Socolovschi, C" uniqKey="Socolovschi C">C Socolovschi</name>
</author>
<author><name sortKey="Mediannikov, O" uniqKey="Mediannikov O">O Mediannikov</name>
</author>
<author><name sortKey="Tall, A" uniqKey="Tall A">A Tall</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reller, Me" uniqKey="Reller M">ME Reller</name>
</author>
<author><name sortKey="Clemens, Eg" uniqKey="Clemens E">EG Clemens</name>
</author>
<author><name sortKey="Schachterle, Se" uniqKey="Schachterle S">SE Schachterle</name>
</author>
<author><name sortKey="Mtove, Ga" uniqKey="Mtove G">GA Mtove</name>
</author>
<author><name sortKey="Sullivan, Dj" uniqKey="Sullivan D">DJ Sullivan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Elbir, H" uniqKey="Elbir H">H Elbir</name>
</author>
<author><name sortKey="Gimenez, G" uniqKey="Gimenez G">G Gimenez</name>
</author>
<author><name sortKey="Sokhna, C" uniqKey="Sokhna C">C Sokhna</name>
</author>
<author><name sortKey="Bilcha, Kd" uniqKey="Bilcha K">KD Bilcha</name>
</author>
<author><name sortKey="Ali, J" uniqKey="Ali J">J Ali</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lescot, M" uniqKey="Lescot M">M Lescot</name>
</author>
<author><name sortKey="Audic, S" uniqKey="Audic S">S Audic</name>
</author>
<author><name sortKey="Robert, C" uniqKey="Robert C">C Robert</name>
</author>
<author><name sortKey="Nguyen, Tt" uniqKey="Nguyen T">TT Nguyen</name>
</author>
<author><name sortKey="Blanc, G" uniqKey="Blanc G">G Blanc</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vial, L" uniqKey="Vial L">L Vial</name>
</author>
<author><name sortKey="Durand, P" uniqKey="Durand P">P Durand</name>
</author>
<author><name sortKey="Arnathau, C" uniqKey="Arnathau C">C Arnathau</name>
</author>
<author><name sortKey="Halos, L" uniqKey="Halos L">L Halos</name>
</author>
<author><name sortKey="Diatta, G" uniqKey="Diatta G">G Diatta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cutler, Sj" uniqKey="Cutler S">SJ Cutler</name>
</author>
<author><name sortKey="Akintunde, Cok" uniqKey="Akintunde C">COK Akintunde</name>
</author>
<author><name sortKey="Moss, J" uniqKey="Moss J">J Moss</name>
</author>
<author><name sortKey="Fukunaga, M" uniqKey="Fukunaga M">M Fukunaga</name>
</author>
<author><name sortKey="Kurtenbach, K" uniqKey="Kurtenbach K">K Kurtenbach</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corpet, F" uniqKey="Corpet F">F Corpet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Socolovschi, C" uniqKey="Socolovschi C">C Socolovschi</name>
</author>
<author><name sortKey="Mediannikov, O" uniqKey="Mediannikov O">O Mediannikov</name>
</author>
<author><name sortKey="Sokhna, C" uniqKey="Sokhna C">C Sokhna</name>
</author>
<author><name sortKey="Tall, A" uniqKey="Tall A">A Tall</name>
</author>
<author><name sortKey="Diatta, G" uniqKey="Diatta G">G Diatta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fenollar, F" uniqKey="Fenollar F">F Fenollar</name>
</author>
<author><name sortKey="Mediannikov, O" uniqKey="Mediannikov O">O Mediannikov</name>
</author>
<author><name sortKey="Socolovschi, C" uniqKey="Socolovschi C">C Socolovschi</name>
</author>
<author><name sortKey="Bassene, H" uniqKey="Bassene H">H Bassene</name>
</author>
<author><name sortKey="Diatta, G" uniqKey="Diatta G">G Diatta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Scott, Jc" uniqKey="Scott J">JC Scott</name>
</author>
<author><name sortKey="Wright, Djm" uniqKey="Wright D">DJM Wright</name>
</author>
<author><name sortKey="Cutler, Sj" uniqKey="Cutler S">SJ Cutler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Dam, Ap" uniqKey="Van Dam A">AP van Dam</name>
</author>
<author><name sortKey="Van Gool, T" uniqKey="Van Gool T">T van Gool</name>
</author>
<author><name sortKey="Wetsteyn, Jc" uniqKey="Wetsteyn J">JC Wetsteyn</name>
</author>
<author><name sortKey="Dankert, J" uniqKey="Dankert J">J Dankert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jiang, J" uniqKey="Jiang J">J Jiang</name>
</author>
<author><name sortKey="Temenak, Jj" uniqKey="Temenak J">JJ Temenak</name>
</author>
<author><name sortKey="Richards, Al" uniqKey="Richards A">AL Richards</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mitani, H" uniqKey="Mitani H">H Mitani</name>
</author>
<author><name sortKey="Talbert, A" uniqKey="Talbert A">A Talbert</name>
</author>
<author><name sortKey="Fukunaga, M" uniqKey="Fukunaga M">M Fukunaga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reye, Al" uniqKey="Reye A">AL Reye</name>
</author>
<author><name sortKey="Arinola, Og" uniqKey="Arinola O">OG Arinola</name>
</author>
<author><name sortKey="Hubschen, Jm" uniqKey="Hubschen J">JM Hübschen</name>
</author>
<author><name sortKey="Muller, Cp" uniqKey="Muller C">CP Muller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sokhna, C" uniqKey="Sokhna C">C Sokhna</name>
</author>
<author><name sortKey="Mediannikov, O" uniqKey="Mediannikov O">O Mediannikov</name>
</author>
<author><name sortKey="Fenollar, F" uniqKey="Fenollar F">F Fenollar</name>
</author>
<author><name sortKey="Bassene, H" uniqKey="Bassene H">H Bassene</name>
</author>
<author><name sortKey="Diatta, G" uniqKey="Diatta G">G Diatta</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Royaume-Uni</li>
<li>Sénégal</li>
<li>Éthiopie</li>
</country>
<region><li>Angleterre</li>
<li>Grand Londres</li>
<li>Provence-Alpes-Côte d'Azur</li>
</region>
<settlement><li>Londres</li>
<li>Marseille</li>
</settlement>
<orgName><li>Université d'Aix-Marseille</li>
</orgName>
</list>
<tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Elbir, Haitham" sort="Elbir, Haitham" uniqKey="Elbir H" first="Haitham" last="Elbir">Haitham Elbir</name>
</region>
<name sortKey="Drancourt, Michel" sort="Drancourt, Michel" uniqKey="Drancourt M" first="Michel" last="Drancourt">Michel Drancourt</name>
<name sortKey="Henry, Mireille" sort="Henry, Mireille" uniqKey="Henry M" first="Mireille" last="Henry">Mireille Henry</name>
<name sortKey="Mediannikov, Oleg" sort="Mediannikov, Oleg" uniqKey="Mediannikov O" first="Oleg" last="Mediannikov">Oleg Mediannikov</name>
<name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name>
<name sortKey="Socolovschi, Cristina" sort="Socolovschi, Cristina" uniqKey="Socolovschi C" first="Cristina" last="Socolovschi">Cristina Socolovschi</name>
</country>
<country name="Sénégal"><noRegion><name sortKey="Diatta, Georges" sort="Diatta, Georges" uniqKey="Diatta G" first="Georges" last="Diatta">Georges Diatta</name>
</noRegion>
<name sortKey="Mediannikov, Oleg" sort="Mediannikov, Oleg" uniqKey="Mediannikov O" first="Oleg" last="Mediannikov">Oleg Mediannikov</name>
<name sortKey="Sokhna, Cheikh" sort="Sokhna, Cheikh" uniqKey="Sokhna C" first="Cheikh" last="Sokhna">Cheikh Sokhna</name>
<name sortKey="Tall, Adama" sort="Tall, Adama" uniqKey="Tall A" first="Adama" last="Tall">Adama Tall</name>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Cutler, Sally J" sort="Cutler, Sally J" uniqKey="Cutler S" first="Sally J." last="Cutler">Sally J. Cutler</name>
</region>
</country>
<country name="Éthiopie"><noRegion><name sortKey="Bilcha, Kassahum D" sort="Bilcha, Kassahum D" uniqKey="Bilcha K" first="Kassahum D." last="Bilcha">Kassahum D. Bilcha</name>
</noRegion>
<name sortKey="Ali, Jemal" sort="Ali, Jemal" uniqKey="Ali J" first="Jemal" last="Ali">Jemal Ali</name>
</country>
<country name="Australie"><noRegion><name sortKey="Campelo, Dayana" sort="Campelo, Dayana" uniqKey="Campelo D" first="Dayana" last="Campelo">Dayana Campelo</name>
</noRegion>
<name sortKey="Barker, Steven C" sort="Barker, Steven C" uniqKey="Barker S" first="Steven C." last="Barker">Steven C. Barker</name>
</country>
</tree>
</affiliations>
</record>
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